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OLYMPUS FV3000 SPECTRAL CONFOCAL MICROSCOPE

The BME Confocal Microscopy Facility in Volker Gall is located on the G (ground) floor inside the BME Suite, Room G094 

Laser scanning microscopy is used in biological research to obtain high-resolution, high-contrast imagery of a sample. Laser microscopes can scan samples point by point, resulting in optical sectioning that can be used to construct precise 3D imagery.
Olympus laser scanning microscopes are designed with a large range of imaging modalities to meet some of the most difficult challenges in the life sciences. Our laser scanning microscopes offer high sensitivity and speed, enabling live cell imaging alongside deep tissue observation.

Using proprietary spectral detection technology, the FV3000 confocal microscope's TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores. 

  • TruSpectral technology achieves up to 3X more light transmission vs. traditional spectral detection technology by implementing the volume phase holographic (VPH) diffraction grating.

  • Offers independently adjustable channels to optimize signal detection for each individual fluorophore. Variable barrier filter mode provides simultaneous four-channel image acquisition with up to sixteen channels in sequential mode.

  • Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals.

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  • X Line objectives offer the widest range of chromatic correction, from 400 nm – 1000 nm, for exceptional color accuracy during multicolor fluorescence imaging

Equipment policy:

1. The PI (Dr. Zhang) has the final decision of how the equipment can be used by inside or outside lab personnel, and under which conditions.

2. Zhang lab members have the priority to reserve the timeslot and use the equipment. In the equipment schedule, Zhang lab members have the right to overwrite the timeslot reserved by non-lab members. Doing so, Zhang lab members should notify the being-overwritten people at least one hour before the timeslot.

3. Other lab members who collaborate with Zhang lab can use this equipment free of charge.

4. Non-lab and non-collaborating people who want to use the equipment needs permission from the PI and, in general, is charged $45 per usage hour.

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To reserve a time slot log into the Google account using the following Excel spread sheet: 

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